The Genome Analysis Services group offers technologies for high-throughput DNA sequencing and genotyping, library construction, and advanced data mining, to enable discovery and validation of molecular genetic markers an single nucleotide polymorphisms (SNPs).

Genome Analysis has applications across the entire spectrum of drug discovery and development, including discovery of genes linked to disease/drug targets, development of DNA-based clinical diagnostics and Pharmacogenomic analysis. In agro sector, it has applications in the development and testing of new seed varieties, including development of markers, development of linkage maps, marker assisted breeding and varietal identification and fingerprinting. Avesthagen RPO further adds value to it by integrating wet-lab work with bioinformatics platform. EST databases, SNP databases, comparative genomics, genome mapping are results of such integration.

In the Pharma sector, genome analysis has applications across the entire spectrum of drug discovery and development, including:

• High throughput sequencing & genotyping
• Discovery and validation of novel drug targets
• Development of DNA-based clinical diagnostics
• Pharmacogenomic analysis of drug response in human clinical trials

Click here to view Selected projects in Pharma sector.

In the agro sector, genome analysis has applications in the development and testing of new seed varieties, including:

• Development of markers
• Development of linkage maps
• Marker assisted breeding
• Varietal Identification and fingerprinting
• Development of genetically modified (GM) crops

With the sequencing of the rice genome, the potential of using genomics for designing crops with desired traits has been recognized. Genes and regions from different varieties - domestic and wild - can be introgressed into desired backgrounds by marker assisted breeding as well as by transformation and thus designer crops can be developed. Avesthagen has been involved in development of markers linked to various traits . We have also been providing services in the area of marker assisted breeding for pyramiding of genes in various crops. Avesthagen has been a pioneer in India in the development of genetically modified crops. We have developed a patented technology for cytoplasmic male sterility in rice and tomatoes and have developed drought tolerant lines of rice. Avesthagen is also engaged in the production of sunflower with nutritional enhanced for an essential fatty acid.

Click here to view Selected projects in Agro sector.

The Unique selling point (USP) of Avesthagen lies in the integration of the wet-lab work with bioinformatics platform to provide a potent solution for the researcher. EST-databases, SNP databases, comparative genomics, genome mapping are the result of such integration

Technologies / Infrastructure

• High Thoughput sequencing, genotyping and genome data mining
  • 1400 sequencing reactions per day generating high quality reads of up to 700 base-pairs.
  • Custom database development / data management / data mining services leading to the identification of novel genes and SNPs.
• DNA library construction
  • Genomic library construction

    - Tiled M. Tuberculosis (strain. H37Rv) BAC library available for   licensing.
    - BAC library of Basmati rice available for licensing
    - BAC library of Schizochytrium available.
     

  • cDNA library construction - full-length and subtractive cDNA libraries
  • cDNA and subtractive libraries of several tissues from several organisms available
• Molecular Genetic Marker Development
  • DNA fingerprinting, marker development and screening, and genetic transformation
  • Development of PCR based markers for traits of choice.
  • Screening for presence of markers in samples